Populating a ListBox from a database in C#

See this tutorial Displaying Data using ASP.NET 2.0 DataList and C#

Take a look at ADO.NET framework but there is a lot of ways to do that...

MSDN

You can use any ORM (such as Linq to SQL, Entity Framework or NHibernate - depending on your needs and the scale of your application) is order to read objects from the database, and then just add the properties of the objects as items to the list box.

prepare the List<Object> for your database records. create the dynamic ListBox control. add control on your page by :

LixtBox lstBox=new ListBox();
lstBox.Datasource=//your List<object>
//Assign datamembers and value members.

Page.Controls.Add(ListBoxControl)

Hope this helps